Alzheimer’s Disease
Alzheimer’s Disease (AD) is marked by the aggregation of **amyloid-beta (Aβ) and tau proteins**, leading to neuronal toxicity. We employ **Time-Correlated Single-Photon Counting Fluorescence Lifetime Imaging Microscopy (TCSPC-FLIM)** to study aggregation in **in vitro, in cellulo, and in vivo models**. Using *Caenorhabditis elegans* as a model organism allows us to track protein misfolding over time, offering insights into disease progression and drug screening.
Intracellular Aβ42 aggregation leads to cellular thermogenesis
CW Chung, AD Stephens, T Konno, E Ward, E Avezov, CF Kaminski, ...
Journal of the American Chemical Society 144 (22), 10034-10041
A unified in vitro to in vivo fluorescence lifetime screening platform yields amyloid β aggregation inhibitors
S Collins, L van Vliet, F Gielen, M Janeček, SW Valladolid, C Poudel, ...
bioRxiv, 2022.03. 28.485913
Structural progression of amyloid-β Arctic mutant aggregation in cells revealed by multiparametric imaging
M Lu, N Williamson, A Mishra, CH Michel, CF Kaminski, A Tunnacliffe, ...
Journal of Biological Chemistry 294 (5), 1478-1487
Imaging Aβ (1–42) fibril elongation reveals strongly polarised growth and growth incompetent states
LJ Young, GSK Schierle, CF Kaminski
Physical Chemistry Chemical Physics 19 (41), 27987-27996
Publications