Key Publications

Stephens, A..D., Kölbel, J., Moons, R., Chung, C..W., Ruggiero, M..T., Mahmoudi, N., Shmool, T..A., McCoy, T..M., Nietlispach, D., Routh, A..F., Sobott, F., Zeitler, J..A. and Schierle, G., Decreased Water Mobility Contributes To Increased α-Synuclein Aggregation. Angew. Chem. Int. Ed., 2022

Chung, C.W., Stephens, A.D., Konno, T., Ward, E., Avezov, Kaminski, C.F., Hassanali, A. A., Kaminski Schierle, G.S. Intracellular Aβ42 aggregation leads to cellular thermogenesis. Journal of the American Chemical Society, 2022

Chung, C.W., Stephens, A.D., Ward, E., Feng, Y., Davis, M.J., Kaminski, C.F., Kaminski Schierle, G.S. Label-Free Characterization of Amyloids and Alpha-Synuclein Polymorphs by Exploiting Their Intrinsic Fluorescence Property. Analytical Chemistry, 2022

Robbins, M., Pisupati, V., Azzarelli, R. et al. Biofunctionalised bacterial cellulose scaffold supports the patterning and expansion of human embryonic stem cell-derived dopaminergic progenitor cells. Stem Cell Res Ther, 2021

Stephens, A.D., Qaisrani, M.N., Ruggiero, M.T., Díaz Mirón, G., Morzan, U.N., González Lebrero, M.C., Jones, S.T.E., Poli, E., Bond, A.D., Woodhams, P.J., Kleist, E.M., Grisanti, L., Gebauer, R., Zeitler, J.A., Credgington, D., Hassanali, A., Kaminski Schierle, G.S. Short hydrogen bonds enhance nonaromatic protein-related fluorescence. PNAS, 2021

Middya, S., Curto, V. F., Fernández-Villegas, A., Robbins, M., Gurke, J., Moonen, E. J. M., Kaminski, G. S., Malliaras, G. G., Microelectrode Arrays for Simultaneous Electrophysiology and Advanced Optical Microscopy. Adv. Sci., 2021

Stephens, A.D., Kölbel, J., Moons, R., Ruggerio, M.T., Mahmoudi, M., Shmool, T.A., McCoy, T.M., Nietlispach, D., Routh, A.F., Sobott, F., Zeitler, J.A. Kaminski Schierle, G.S. The role of water mobility in protein misfolding. bioRxiv 2021.01.06.425575, 2021

Robbins, M., Siddiqui, O., Fuchsberger, T., Goodfellow, G., Paulsen, O., Kaminski, C.F., Euser, T. and Schierle, G.S.K. OptoGenie: an open-source device for the optogenetic stimulation of cells. Journal of Open Hardware, 5(1), p.2. , 2021

Chung C.W., Kaminski Schierle G.S., Intracellular thermometry at the micro‐/nanoscale and its potential application to study protein aggregation related to neurodegenerative diseases, ChemBioChem, 2020

Lu M, van Tartwijk FW, Qiaojin Lin J, Nijenhuis W, Parutto P, Fantham M, Christensen CN, Avezov E, Holt CE, Tunnacliffe A, Holcman D, Kapitein L, Kaminski Schierle GS, Kaminski CF, The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes, Science Advances, Vol 6, No. 51, 2020

Stephens AD, Matak-Vinkovic D, Fernandez-Villegas A, Kaminski-Schierle GS, Purification of Recombinant α-synuclein: A Comparison of Commonly Used Protocols, ASC Biochem 59, 48, 4563–4572, 2020

Stephens AD, Lu M, Fernandez-Villegas A, Kaminski-Schierle GS, Fast Purification of Recombinant Monomeric Amyloid-β from E. coli and Amyloid-β-mCherry Aggregates from Mammalian Cells, ACS Chem. Neurosci. 2020

Stephens AD, Zacharopoulou M, Moons R, Fusco G, Seetaloo N, Chiki A, Hooper PJ, Mela I, Lashuel HA, Philips JJ, De Simone A, Sobott FD, Kaminski Schierle GS, Extent of N-terminus exposure of monomeric alpha-synuclein determines its aggregation propensity, Nat Commun 11, 2820 (2020)

Lautenschläger J., Wagner-Valladolid S., Stephens AD., Fernández-Villegas A., Hockings C., Mishra A., Manton J.D., Fantham M.J., Lu M., Rees E.J., Kaminski1 C.F., and Kaminski Schierle G.S., Intramitochondrial proteostasis is directly coupled to α-synuclein and amyloid β 1-42 pathologies, J. Biol. Chem. jbc.RA119.011650 (2020)

Hockings C., Poudel C., Feeney K.A., Novo C.L., Hamouda M.S., Mela I., Fernandez-Antoran D., Vallejo-Ramirez P.P., Rugg-Gunn P.J., Chalut K., Kaminski C.F., Kaminski-Schierle G.S., Illuminating chromatin compaction in live cells and fixed tissues using SiR-DNA fluorescence lifetime, (Preprint), bioRXiv

Stephens AD, Kaminski Schierle GS, The role of water in amyloid aggregation kinetics., Curr Opin Struct Biol. 2019 Jul 9;58:115-123.

Lautenschläger J, Kaminski Schierle GS., Mitochondrial degradation of amyloidogenic proteins — A new perspective for neurodegenerative diseases, Prog Neurobiol. 2019 Oct;181:101660.

Huang C, Wagner-Valladolid S, Stephens AD, Jung R, Poudel C, Sinnige T, Lechler MC, Schlörit N, Lu M, Laine RF, Michel CH, Vendruscolo M, Kaminski CF, Kaminski Schierle GS, David DC., Intrinsically aggregation-prone proteins form amyloid-like aggregates and contribute to tissue aging in Caenorhabditis elegans., eLife 2019; 8: e43059,.

Laine RF, Sinnige T, Ma KY, Haack AJ, Poudel C, Gaida P, Curry N, Perni M, Nollen EAA, Dobson CM, Vendruscolo M, Kaminski Schierle GS, Kaminski CF., Fast Fluorescence Lifetime Imaging Reveals the Aggregation Processes of α-Synuclein and Polyglutamine in Aging Caenorhabditis elegans., ACS Chem Biol. 2019 Jul 19;14(7):1628-1636

Lu M, Banetta L, Young LJ, Smith EJ, Bates GP, Zaccone A, Kaminski Schierle GS, Tunnacliffe A, Kaminski CF. “Live-cell super-resolution microscopy reveals a primary role for diffusion in polyglutamine-driven aggresome assembly” J Biol Chem, (2019), 294(1):257-268. doi: 10.1074/jbc.RA118.003500.

Stephens AD, Zacharopoulou M, Kaminski Schierle GS. ”The Cellular Environment Affects Monomeric α-Synuclein Structure” Trends Biochem Sci. (2018). pii: S0968-0004(18)30246-9. doi: 10.1016/j.tibs.2018.11.005.

Lu M, Williamson N, Mishra A, Michel CH, Kaminski CF, Tunnacliffe A, Kaminski Schierle GS “Structural progression of amyloid-β Arctic mutant aggregation in cells revealed by multi-parametric imaging” J Biol Chem, (2018) Nov 30. doi: 10.1074/jbc.RA118.004511

Stephens A D, Nespovitaya N, Zacharopoulou M, Kaminski C F, Phillips J J, Kaminski Schierle G S “Different Structural Conformers of Monomeric α-Synuclein Identified after Lyophilizing and Freezing” Analytical Chemistry, (2018), 90 (11), 6975-6983 doi:10.1021/acs.analchem.8b01264

Qamar S, Wang G, Randle S J, Simone Ruggeri F, Varela J A, Lin J Q, Phillips E C, Miyashita A, Williams D, Ströhl F, Meadows W, Ferry R, Dardov V J, Tartaglia G G, Farrer L A, Kaminski Schierle G S, Kaminski C F, Holt C E, Fraser P E, Schmitt-Ulms G, Klenerman D, Knowles T, Vendruscolo M, St George-Hyslop P, "FUS Phase Separation Is Modulated by a Molecular Chaperone and Methylation of Arginine Cation-π Interactions", Cell, (2018), 173, (3), 720-734. doi.org/10.1016/j.cell.2018.03.056 | summary

Lautenschläger J, Stephens AD, Fusco G, Ströhl F, Curry N, Zacharopoulou M, Michel CH, Laine R, Nespovitaya N, Fantham M, Pinotsi D, Zago W, Fraser P, Tandon A, St George-Hyslop P, Rees E, Phillips JJ, De Simone A, Kaminski CF, Kaminski Schierle GS, "C-terminal calcium binding of α-synuclein modulates synaptic vesicle interaction", Nature Communications, (2018), 9 712. doi:10.1038/s41467-018-03111-4 | summary

Lautenschläger J, Kaminski CF, Kaminski Schierle GS, "α-Synuclein – Regulator of Exocytosis, Endocytosis, or Both?", Trends in Cell Biology (2017). doi:10.1016/j.tcb.2017.02.002

ens AD, Lu M, Fernandez-Villegas A, Kaminski-Schierle GS, Fast Purification of Recombinant Monomeric Amyloid-β from E. coli and Amyloid-β-mCherry Aggregates from Mammalian Cells, ACS Chem. Neurosci. 2020