Our preprint 'The mobility of water influences protein misfolding' shows ions influence water motions which are inextricably linked to protein dynamics and therefore misfolding propensity.
Can the presence of different ions and water mobility in cells influence disease outcome?
2 Feb 2021
Chyi's review on intracellular thermometry probes and their application to study thermodynamic properties of cells and energy flow in protein aggregation is published in ChemBioChem and featured as the technology of the month in Trends in Biochemical Sciences.
We highlight different nanoscale thermoprobes that have enabled intracellular temperature gradients to be visualised. Intracellular thermometry is proposed as a tool to quantify energetics involved in amyloid-beta 42 aggregation, a hallmark of Alzheimers' disease.
6 Jan 2021
A new mechanism on how endoplasmic reticulum reshapes is revealed by a combinatorial method of super-resolution imaging, optogenetic tools and machine-learning from LAG-MNG:
The endoplasmic reticulum (ER) is dynamically regulated by lysosomes by VAPA dependent membrane contacts.
In neuronal axons, lysosomes drive ER tubules to move along axons thus connect ER fragments to be a continuous membrane structure.
Find our paper here.
17 Dec 2020
Our new publication 'Purification of Recombinant α-synuclein: A Comparison of Commonly Used Protocols'shows our choice of purification method for a-syn influences the outcome of downstream assays
We therefore wonder whether the choice of purification method e.g. 'harsh' treatments such as boiling, can influence the dynamic ensemble of the monomeric protein, changing the conformational distribution, and influence downstream assays, such as cell response or aggregation rate.
29 Nov 2020
A new paper on Αβ purification led by Amberley Stephens is out now.
We provide a method for the purification of recombinant label-free amyloid-β using commercially available reagents and without the need for a freeze drying step which induces oligomerisation.
1 Oct 2020
Many congratulations to our group leader, Gabi, on her promotion to Reader!
27 June 2020
We are very excited to share our latest research published in Nature Communications on alpha-synuclein conformational dynamics!
We investigated the monomeric conformation of aSyn in different conditions and found that the more exposed the N-terminus of the protein becomes, the more aggregation-prone it becomes.
Sequence alterations such as the presence of post-translational modifications and familial point mutations that lead to early-onset Parkinson's disease also lead to differing exposure of the N-terminus, linked to aggregation propensity.
This paper is led by Amberley Stephens and Maria Zacharopoulou and is a result of a big collaboration cohort. Many congratulations to all!
04 June 2020
Our new article sheds light on the coupling between mitochondrial impairment and α-synuclein pathology!
We show that protein homeostasis in mitochondria plays a major role in α-synuclein aggregation, as a disruption of intramitochondrial proteases leads to aggravated α-synuclein seeding.
The paper is led by Janin Lautenshläger, and includes work performed by many LAG and MNG members. Many congratulations to all!
8 May 2020
The article introduces a new method based on fluorescence lifetime to measure chromatin compaction in any cell, live or fixed, without genetic modification. It is a result of the collaboration between members of the Laser Analytics Group and the Molecular Neuroscience Group. Many congratulations to all who contributed!
3 May 2020
Ioanna Mela's article on DNA-origami as an antimicrobial delivery agent is published in Angewandte Chemie.
The paper is led by the Laser Analytics Group's postdoc Ioanna Mela and includes collaborations with MNG.
Many congratulations to authors: Ioanna Mela, Pedro Vallejo-Ramirez, Stanislaw Makarchuk and Clemens Kaminski!
15 April 2020
Despite the national quarantine we continue our work remotely - more new exciting projects on the way!
Miranda Robbins has passed her viva!!
Congratulations to Dr Miranda Robbins for passing her PhD viva!
16 December 2019
Philippa Hooper has passed her viva!!
Congratulations to Dr Philippa Hooper who passed her viva in only 2 hours!
13 September 2019
Researchers in the Molecular Neuroscience Group have found that excess levels of calcium in brain cells may lead to the formation of toxic clusters that are the hallmark of Parkinson’s disease.
Read more here.
19 February 2019